Neural Engineering

  • Neural Engineering > Optogenetics

What is Optogenetics?

Optogenetics most commonly refers to a biological technique that involves the use of light to control neurons that have been genetically modified to express light-sensitive ion channels. As such, optogenetics is a neuromodulation method that uses a combination of techniques from optics and genetics to control the activities of individual neurons in living tissue—even within freely-moving animals. In some usages, optogenetics also refers to optical monitoring of neuronal activity and control of biochemical pathways in non-neuronal cells, although these research activities preceded the use of light-sensitive ion channels in neurons.

Research 1 > Cell type specific conjugating method using genetically encoded AviTag expressed optogenetic opsins

그림1. AviTag-ChR2-EYFP lentiviral vector의 제작 및 biotin coated 655 nm Qdot 을 이용한 direct conjugating method

그림2. vidn(SAv/TAv) coated fluorescent 프로브를 이용한 In-direct conjugating method

  • Publications

[4] S Lee, BE Kang, M Kim, Y.-K Song, BJ Baker, “Variable Consequences of Membrane Targeting Motifs for Genetically Encoded Voltage Indicators”, Biophysical journal, 2019.

[3] S. Lee Y.-K. Song, BJ Baker, "Engineering Photoactivatability in Genetically Encoded Voltage and pH Indicators", Frontiers in cellular neuroscience, 2019.

[2] S. Lee, T. Geiller, A. Jung, R. Nakajima, Y-K. Song, B.J. Baker, "Improving a genetically encoded voltage indicator by modifying the cytoplasmic charge composition," Scientific Reports, 2017.

[1] S. Lee, H.H. Piao, M. Sepheri-Rad, A. Jung, U. Sung, Y.K. Song, B.J. Baker, "Imaging Membrane Potential with Two Types of Genetically Encoded Fluorescent Voltage Sensors," Journal of Visualized Experiments, 2016.